Reference : Interleukin-27 displays interferon-gamma-like functions in human hepatoma cells and h...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/14733
Interleukin-27 displays interferon-gamma-like functions in human hepatoma cells and hepatocytes.
English
Bender, Herdis [> >]
Wiesinger, Monique mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Nordhoff, Carolin [> >]
Schoenherr, Caroline [> >]
Haan, Claude mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Ludwig, Stephan [> >]
Weiskirchen, Ralf [> >]
Kato, Nobuyuki [> >]
Heinrich, Peter C. [> >]
Haan, Serge mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
2009
Hepatology (Baltimore, Md.)
50
2
585-91
Yes (verified by ORBilu)
International
0270-9139
1527-3350
United States
[en] Animals ; Antimicrobial Cationic Peptides/metabolism ; Carcinoma, Hepatocellular/metabolism ; Cell Line, Tumor ; Fibrinogen/metabolism ; Gene Expression Regulation ; Hepatocytes/metabolism ; Humans ; Interferon-gamma/metabolism ; Interleukins/immunology/metabolism ; Liver Neoplasms/metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; STAT1 Transcription Factor/metabolism ; STAT3 Transcription Factor/metabolism ; Virus Replication
[en] Interleukin-27 (IL-27) is a cytokine belonging to the IL-6/IL-12 cytokine family. It is secreted by antigen-presenting cells, strongly acts on T cells, and also stimulates innate immune cells. In most studies, the effects of IL-27 on T cells were investigated; however, not much is known about possible effects of IL-27 on other cell types. IL-27 signals via the common IL-6-type cytokine receptor chain gp130 and the IL-27-specific chain WSX-1. Given the importance of gp130 in regulating liver responses such as the acute phase response or liver regeneration, we investigated whether IL-27 could also have a function in liver cells. We find that IL-27 stimulates hepatoma cells and hepatocytes by inducing a sustained signal transducer and activator of transcription (STAT)1 and STAT3 activation. Whereas the STAT3 mediated responses to IL-27 (gamma-fibrinogen and hepcidin induction) are not detectable, we observe an interferon-gamma (IFN-gamma)-like STAT1 response leading to the induction of interferon-regulated proteins such as STAT1, STAT2, interferon response factor (IRF)-1, IRF-9, myxovirus resistance A and guanylate binding protein 2. CONCLUSION: Our study provides evidence for a function of IL-27 in hepatoma cells and hepatocytes and shows that IL-27 responses are not restricted to the classical immune cells. Our results suggest that IL-27 exerts IFN-like functions in liver cells and that it can contribute to the antiviral response in these cells.
http://hdl.handle.net/10993/14733
10.1002/hep.22988

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