Reference : Detection of activated STAT species using electrophoretic mobility shift assay (EMSA)...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/10993/10694
Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents.
English
Haan, Serge mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Haan, Claude mailto [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
2013
Methods in Molecular Biology (Clifton, N.J.)
967
147-59
Yes (verified by ORBilu)
International
1064-3745
United States
[en] Here we describe the preparation of nuclear extracts and the electrophoretic mobility shift assay (EMSA) for the detection of STAT species. We use the method for the investigation of STAT1 and STAT3 homo- and heterodimers and show how the preparation of the extracts can influence the distribution of the STAT species observed in the EMSA. We show that detergents can massively influence the STAT dimer distribution. Although it is unclear whether they primarily interfere with STAT DNA binding and/or whether they break up or further oligomerize STATs, the observation may also have an impact on the results of other techniques performed with detergent-containing cell lysates (e.g., coimmunoprecipitations of STATs with other proteins).
http://hdl.handle.net/10993/10694
10.1007/978-1-62703-242-1_10

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Limited access
Haan_Methods in Molecular Biology 2013_967_147-159.pdfAuthor postprint338.62 kBRequest a copy

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.