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See detail3D organotypic cultures of human HepaRG cells: a tool for in vitro toxicity studies.
Gunness, Patrina; Mueller, Daniel; Shevchenko, Valery et al

in Toxicological sciences : an official journal of the Society of Toxicology (2013), 133(1), 67-78

Drug-induced human hepatotoxicity is difficult to predict using the current in vitro systems. In this study, long-term 3D organotypic cultures of the human hepatoma HepaRG cell line were prepared using a ... [more ▼]

Drug-induced human hepatotoxicity is difficult to predict using the current in vitro systems. In this study, long-term 3D organotypic cultures of the human hepatoma HepaRG cell line were prepared using a high-throughput hanging drop method. The organotypic cultures were maintained for 3 weeks and assessed for (1) liver specific functions, including phase I enzyme and transporter activities, (2) expression of liver-specific proteins, and (3) responses to three drugs (acetaminophen, troglitazone, and rosiglitazone). Our results show that the organotypic cultures maintain high liver-specific functionality during 3 weeks of culture. The immunohistochemistry analyses illustrate that the organotypic cultures express liver-specific markers such as albumin, CYP3A4, CYP2E1, and MRP-2 throughout the cultivation period. Accordingly, the production rates of albumin and glucose, as well as CYP2E1 activity, were significantly higher in the 3D versus the 2D cultures. Toxicity studies show that the organotypic cultures are more sensitive to acetaminophen- and rosiglitazone-induced toxicity but less sensitive to troglitazone-induced toxicity than the 2D cultures. Furthermore, the EC50 value (2.7mM) for acetaminophen on the 3D cultures was similar to in vivo toxicity. In summary, the results from our study suggest that the 3D organotypic HepaRG culture is a promising in vitro tool for more accurate assessment of acute and also possibly for chronic drug-induced hepatotoxicity. [less ▲]

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See detail3D organotypic HepaRG cultures as in vitro model for acute and repeated dose toxicity studies.
Mueller, Daniel; Kramer, Lisa; Hoffmann, Esther et al

in Toxicology in vitro : an international journal published in association with BIBRA (2014), 28(1), 104-12

Predictive in vitro models alternative to in vivo animal will have a significant impact in toxicology. Conventional 2D models do not reflect the complexity of a 3D organ resulting in discrepancies between ... [more ▼]

Predictive in vitro models alternative to in vivo animal will have a significant impact in toxicology. Conventional 2D models do not reflect the complexity of a 3D organ resulting in discrepancies between experimental in vitro and in vivo data. Using 3D HepaRG organotypic cultures we tested four drugs (aflatoxin B1, amiodarone, valproic acid and chlorpromazine) for toxic effects and compared the results with 2D HepaRG and HepG2 cultures. We show that 3D HepaRG cultures are more sensitive than the other tested cultures to aflatoxin B1 which is only toxic upon metabolic activation in the liver. We observed that CYP3A4 activity is higher in the 3D HepaRG cultures compared to the 2D HepaRG cultures. Furthermore, we investigated repeated dose toxicity of chlorpromazine and assessed its effects on glucose and lactate metabolism. Sub-toxic concentrations of chlorpromazine induced significant metabolic changes in both 2D and 3D HepaRG cultures upon acute and repeated dose (3 doses) exposure. In summary, our data support the hypothesis that 3D cell culture models better mimic the in vivo tissue and improve cellular functionality. The 3D HepaRG organotypic cultures represent a high throughput system for drug toxicity screening. This system is therefore a promising tool in preclinical testing of human relevance which can allow reducing and/or replacing animal testing for drug adverse effects. [less ▲]

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See detail3D pose estimation based on planar object tracking for UAVs control
Mondragon, Ivan F.; Campoy, Pascual; Martinez, Carol et al

in Proccedings of IEEE International Conference on Robotics and Automation (ICRA) (2010)

This article presents a real time Unmanned Aerial Vehicles UAVs 3D pose estimation method using planar object tracking, in order to be used on the control system of a UAV. The method explodes the rich ... [more ▼]

This article presents a real time Unmanned Aerial Vehicles UAVs 3D pose estimation method using planar object tracking, in order to be used on the control system of a UAV. The method explodes the rich information obtained by a projective transformation of planar objects on a calibrated camera. The algorithm obtains the metric and projective components of a reference object (landmark or helipad) with respect to the UAV camera coordinate system, using a robust real time object tracking based on homographies. The algorithm is validated on real flights that compare the estimated data against that obtained by the inertial measurement unit IMU, showing that the proposed method robustly estimates the helicopter's 3D position with respect to a reference landmark, with a high quality on the position and orientation estimation when the aircraft is flying at low altitudes, a situation in which the GPS information is often inaccurate. The obtained results indicate that the proposed algorithm is suitable for complex control tasks, such as autonomous landing, accurate low altitude positioning and dropping of payloads. [less ▲]

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See detail3D Printing: The Next 5 years by Claude Wolf – Into the Future engineered by the Zs
Wolf, Claude UL

E-print/Working paper (2017)

3D Printing: The Next 5 years by Claude Wolf – Into the Future engineered by the Zs The future of 3D Printing or Additive Manufacturing [AM] will be driven by the next generation of engineers who are just ... [more ▼]

3D Printing: The Next 5 years by Claude Wolf – Into the Future engineered by the Zs The future of 3D Printing or Additive Manufacturing [AM] will be driven by the next generation of engineers who are just about to qualify, the Z generation (1994-2010). Do we, educators, know this Z generation? Do we know what motivates them? The Z generation is a generation of digital natives interacting with PCs, iPads, smartphones and the internet since birth, a pragmatic and short term orientated generation. Our prejudice brands them as unprepared and unmotivated, with their 10 second attention spans, but in reality, they have already proven they are creative, determined and hardworking as well as team oriented, when they are given the opportunity. They have understood that 3D printing offers them this opportunity. [less ▲]

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See detailA 3D shear material damping model for man-made vibrations of the ground
Macijauskas, Darius UL; Van Baars, Stefan UL

in 13th Baltic Sea Region Geotechnical Conference (2016, September)

Man-made vibrations from different types of sources are usually measured on the surface of the ground or building. The measured signal is always the superposition of all travelling basic waves. For a ... [more ▼]

Man-made vibrations from different types of sources are usually measured on the surface of the ground or building. The measured signal is always the superposition of all travelling basic waves. For a homogeneous half space there are three basic waves – the Compressional (P-wave), Shear (S-wave) and Rayleigh wave (R-wave). Depending on the measuring equipment, only the accelerations or velocities in time of the superposed wave can be measured, but not the distribution of the individual basic waves. Additional problems are that each of the basic waves has its own velocity, besides the body and surface waves have different attenuation laws. By using the rules of superposition of harmonic waves and also the propagation laws of the P-, S- and R-waves, it should be theoretically possible to split the measured superposed signal into the basic waves, because mathematically a system of equations can be assembled which describes the displacements at multiple measuring points in time. In this paper this problem has been solved for a homogenous, elastic and isotropic soil, which is disturbed by a harmonically oscillating disc on the surface. A numerical simulation was performed using a finite element method. The displacements in time were recorded in 10 points on the surface and a system of superposed equations was assembled and solved. The findings prove that each of the three basic waves has its own phase shift with the source, something which was not known before. [less ▲]

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See detail3D Skeleton based Head Detection and Tracking using Range Images
Devarakota, Pandu UL; Castillo-Franco, Marta; Ginhoux, Romuald et al

in IEEE Transactions on Vehicular Technology (2009), 58(8), 4064-4077

Vision-based 3-D head detection and tracking systems have been studied in several applications like video surveillance, face-detection systems, and occupant posture analysis. In this paper, we present the ... [more ▼]

Vision-based 3-D head detection and tracking systems have been studied in several applications like video surveillance, face-detection systems, and occupant posture analysis. In this paper, we present the development of a topology-based framework using a 3-D skeletal model for the robust detection and tracking of a vehicle occupant's head position from low-resolution range image data for a passive safety system. Unlike previous approaches to head detection, the proposed approach explores the topology information of a scene to detect the position of the head. Among the different available topology representations, the Reeb graph technique is chosen and is adapted to low-resolution 3-D range images. Invariance of the graph under rotations is achieved by using a Morse radial distance function. To cope with the particular challenges such as the noise and the large variations in the density of the data, a voxel neighborhood connectivity notion is proposed. A multiple-hypothesis tracker (MHT) with nearest-neighbor data association and Kalman filter prediction is applied on the endpoints of the Reeb graph to select and filter the correct head candidate out of Reeb graph endpoints. A systematic evaluation of the head detection framework is carried out on full-scale experimental 3-D range images and compared with the ground truth. It is shown that the Reeb graph topology algorithm developed herein allows the correct detection of the head of the occupant with only two head candidates as input to the MHT. Results of the experiments demonstrate that the proposed framework is robust under the large variations of the scene. The processing requirements of the proposed approach are discussed. It is shown that the number of operations is rather low and that real-time processing requirements can be met with the proposed method. [less ▲]

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See detail3D Time-Of-Flight Camera System and Position/Orientation Calibration Method Therefor
Garcia Becerro, Frederic UL; Grandidier, Frederic; Mirbach, Bruno et al

Patent (2011)

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See detail3D visualization of archaeological uncertainty
Sifniotis, M.; Jackson, B. J. C.; Mania, K. et al

in Proc. ACM Symposium on Applied Perception (2010)

By uncertainty, we define an archaeological expert's level of confidence in an interpretation deriving from gathered evidence. Archaeologists and computer scientists have urged caution in the use of 3D ... [more ▼]

By uncertainty, we define an archaeological expert's level of confidence in an interpretation deriving from gathered evidence. Archaeologists and computer scientists have urged caution in the use of 3D for archaeological reconstructions because the availability of other possible hypotheses is not always being acknowledged. This poster presents a 3D visualization system of archaeological uncertainty. [less ▲]

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See detail3D-Formanalyse von retroperitonealen Tumoren im Kindesalter: entwicklung einer statistischen 2D/3D-Methodik und Anwendung dieser mit MRT-Datensätzen
Giebel, Stefan Markus; Nourkami, N.; Leuschner, I. et al

Scientific Conference (2010, September 18)

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See detail§ 4 WpHG zur Zuständigkeit der Bundesanstalt für Finanzdienstleistungsaufsicht, Kapitalmarktrechtskommentar
Zetzsche, Dirk Andreas UL

Book published by Schwark/Zimmer - 4. Auflage (2010)

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See detail4-Amino-1,2,4-triazole: Playing a key role in the chemical deposition of Cu–In–Ga metal layers for photovoltaic applications.
Berner, Ulrich; Widenmeyer, Markus; Engler, Patrick et al

in Thin Solid Films (2014)

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See detail4.2 Social Dynamics Metrics-Working Group Report
Benenson, Zinaida; Bleikertz, Sören; Foley, Simon N. et al

in Socio-Technical Security Metrics (2015)

Individuals continually interact with security mechanisms when performing tasks in everyday life. These tasks may serve personal goals or work goals, be individual or shared. These interactions can be ... [more ▼]

Individuals continually interact with security mechanisms when performing tasks in everyday life. These tasks may serve personal goals or work goals, be individual or shared. These interactions can be influenced by peers and superiors in the respective environments (workplace, home, public spaces), by personality traits of the users, as well as by contextual constraints such as available time, cognitive resources, and perceived available effort. All these influencing factors, we believe, should be considered in the design, implementation and maintenance of good socio-technical security mechanisms. Therefore, we need to observe reliable socio-technical data, and then transform them into meaningful and helpful metrics for user interactions and influencing factors. More precisely, there are three main questions that the group discussed: 1. What data do we need to observe and what of this data we actually can observe and measure? 2. How can we observe and measure? 3. What can we do with the results of the observations? [less ▲]

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See detail49. Thierry Revol: Représentation du sacré dans les textes dramatiques des XIe-XIIIe siècles en France
Cicotti, Claudio UL

in Zeitschrift für Romanische Philologhie (2002), 118(4), 744-745

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See detail4D Biology for health and disease
Abrahams, J. P.; Apweiler, R.; Balling, Rudi UL et al

in New Biotechnology (2011), 28(4), 291-293

The "4D Biology Workshop for Health and Disease", held on 16-17th of March 2010 in Brussels, aimed at finding the best organising principles for large-scale proteomics, interactomics and structural ... [more ▼]

The "4D Biology Workshop for Health and Disease", held on 16-17th of March 2010 in Brussels, aimed at finding the best organising principles for large-scale proteomics, interactomics and structural genomics/biology initiatives, and setting the vision for future high-throughput research and large-scale data gathering in biological and medical science. Major conclusions of the workshop include the following. (i) Development of new technologies and approaches to data analysis is crucial. Biophysical methods should be developed that span a broad range of time/spatial resolution and characterise structures and kinetics of interactions. Mathematics, physics, computational and engineering tools need to be used more in biology and new tools need to be developed. (ii) Database efforts need to focus on improved definitions of ontologies and standards so that system-scale data and associated metadata can be understood and shared efficiently. (iii) Research infrastructures should play a key role in fostering multidisciplinary research, maximising knowledge exchange between disciplines and facilitating access to diverse technologies. (iv) Understanding disease on a molecular level is crucial. System approaches may represent a new paradigm in the search for biomarkers and new targets in human disease. (v) Appropriate education and training should be provided to help efficient exchange of knowledge between theoreticians, experimental biologists and clinicians. These conclusions provide a strong basis for creating major possibilities in advancing research and clinical applications towards personalised medicine. [less ▲]

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See detail4th Conference on Poisson Geometry and related fields
Poncin, Norbert UL

Report (2004)

Speakers' abstracts and list of participants of the International Conference on 'Poisson Geometry and related fields' organized in June 2004 at the University of Luxembourg by Norbert Poncin

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See detail5 Figures de l’Histoire des idées européennes ou la recherche de la Communauté politique
Poirier, Philippe UL

in Arjakovsky, Antoine (Ed.) Histoire de la conscience européenne (2016)

Il ne s’agit pas de produire un récit exhaustif ou apologétique ou exclusif de la conscience européenne. Il s’agit de rendre visible la réalité mouvante d’une conscience européenne qui s’affirme de plus ... [more ▼]

Il ne s’agit pas de produire un récit exhaustif ou apologétique ou exclusif de la conscience européenne. Il s’agit de rendre visible la réalité mouvante d’une conscience européenne qui s’affirme de plus en plus en Europe. Pour n’avoir aucun lieu où se penser et où se refléter, celle-ci est bien souvent abandonnée à elle-même au point de douter de sa réalité propre. Mais les enjeux politiques, économiques et culturels de l’Europe politique sont tels que l’Union européenne ne pourra plus se permettre trop longtemps de se passer de récit commun. [less ▲]

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See detail5-Carboxy-8-hydroxyquinoline is a Broad Spectrum 2-Oxoglutarate Oxygenase Inhibitor which Causes Iron Translocation.
Hopkinson, Richard J.; Tumber, Anthony; Yapp, Clarence et al

in Chemical Science (2013), 4(8), 3110-3117

2-Oxoglutarate and iron dependent oxygenases are therapeutic targets for human diseases. Using a representative 2OG oxygenase panel, we compare the inhibitory activities of 5-carboxy-8-hydroxyquinoline ... [more ▼]

2-Oxoglutarate and iron dependent oxygenases are therapeutic targets for human diseases. Using a representative 2OG oxygenase panel, we compare the inhibitory activities of 5-carboxy-8-hydroxyquinoline (IOX1) and 4-carboxy-8-hydroxyquinoline (4C8HQ) with that of two other commonly used 2OG oxygenase inhibitors, N-oxalylglycine (NOG) and 2,4-pyridinedicarboxylic acid (2,4-PDCA). The results reveal that IOX1 has a broad spectrum of activity, as demonstrated by the inhibition of transcription factor hydroxylases, representatives of all 2OG dependent histone demethylase subfamilies, nucleic acid demethylases and gamma-butyrobetaine hydroxylase. Cellular assays show that, unlike NOG and 2,4-PDCA, IOX1 is active against both cytosolic and nuclear 2OG oxygenases without ester derivatisation. Unexpectedly, crystallographic studies on these oxygenases demonstrate that IOX1, but not 4C8HQ, can cause translocation of the active site metal, revealing a rare example of protein ligand-induced metal movement. [less ▲]

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See detail5. Changes in the stratification process in Israel, 1995--2008
Blank, Carmel; Bar-Haim, Eyal UL; Shavit, Yossi

in Education, Occupation and Social Origin: A Comparative Analysis of the Transmission of Socio-Economic Inequalities (2016)

Detailed reference viewed: 15 (1 UL)