References of "Tapia, Natalia"
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See detailInduced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain
Hemmer, Kathrin UL; Zhang, Mingyue; van Wuellen, Thea et al

in Stem Cell Reports (2014)

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See detailDirect reprogramming of fibroblasts into neural stem cells by defined factors.
Han, Dong Wook; Tapia, Natalia; Hermann, Andreas et al

in Cell Stem Cell (2012), 10(4), 465-72

Recent studies have shown that defined sets of transcription factors can directly reprogram differentiated somatic cells to a different differentiated cell type without passing through a pluripotent state ... [more ▼]

Recent studies have shown that defined sets of transcription factors can directly reprogram differentiated somatic cells to a different differentiated cell type without passing through a pluripotent state, but the restricted proliferative and lineage potential of the resulting cells limits the scope of their potential applications. Here we show that a combination of transcription factors (Brn4/Pou3f4, Sox2, Klf4, c-Myc, plus E47/Tcf3) induces mouse fibroblasts to directly acquire a neural stem cell identity-which we term as induced neural stem cells (iNSCs). Direct reprogramming of fibroblasts into iNSCs is a gradual process in which the donor transcriptional program is silenced over time. iNSCs exhibit cell morphology, gene expression, epigenetic features, differentiation potential, and self-renewing capacity, as well as in vitro and in vivo functionality similar to those of wild-type NSCs. We conclude that differentiated cells can be reprogrammed directly into specific somatic stem cell types by defined sets of specific transcription factors. [less ▲]

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