References of "Schwamborn, Jens Christian 50003060"
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See detailSingle-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids
Smith, Lisa M.; Magni, Stefano UL; Grzyb, Kamil UL et al

E-print/Working paper (2019)

Human stem cell-derived organoids have great potential for modelling physiological and pathological processes. They recapitulate in vitro the organisation and function of a respective organ or part of an ... [more ▼]

Human stem cell-derived organoids have great potential for modelling physiological and pathological processes. They recapitulate in vitro the organisation and function of a respective organ or part of an organ. Human midbrain organoids (hMOs) have been described to contain midbrain-specific dopaminergic neurons that release the neurotransmitter dopamine. However, the human midbrain contains also additional neuronal cell types, which are functionally interacting with each other. Here, we analysed hMOs at high-resolution by means of single-cell RNA-sequencing (scRNA-seq), imaging and electrophysiology to unravel cell heterogeneity. Our findings demonstrate that hMOs show essential neuronal functional properties as spontaneous electrophysiological activity of different neuronal subtypes, including dopaminergic, GABAergic, and glutamatergic neurons. Recapitulating these in vivo features makes hMOs an excellent tool for in vitro disease phenotyping and drug discovery. [less ▲]

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See detailAutomated microfluidic cell culture of stem cell derived dopaminergic neurons
Kane, Khalid; Lucumi Moreno, Edinson; Hachi, Siham et al

in Scientific Reports (2019)

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See detailModeling Parkinson’s disease in midbrain-like organoids
Smits, Lisa UL; Reinhardt, Lydia; Reinhardt, Peter et al

in NPJ Parkinson's Disease (2019)

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See detail3D Cultures of Parkinson's Disease‐Specific Dopaminergic Neurons for High Content Phenotyping and Drug Testing
Bolognin, Silvia UL; Fossépré, Marie; Qing, Xiaobing et al

in Advanced Science (2018)

Parkinson's disease (PD)‐specific neurons, grown in standard 2D cultures, typically only display weak endophenotypes. The cultivation of PD patient‐specific neurons, derived from induced pluripotent stem ... [more ▼]

Parkinson's disease (PD)‐specific neurons, grown in standard 2D cultures, typically only display weak endophenotypes. The cultivation of PD patient‐specific neurons, derived from induced pluripotent stem cells carrying the LRRK2‐G2019S mutation, is optimized in 3D microfluidics. The automated image analysis algorithms are implemented to enable pharmacophenomics in disease‐relevant conditions. In contrast to 2D cultures, this 3D approach reveals robust endophenotypes. High‐content imaging data show decreased dopaminergic differentiation and branching complexity, altered mitochondrial morphology, and increased cell death in LRRK2‐G2019S neurons compared to isogenic lines without using stressor agents. Treatment with the LRRK2 inhibitor 2 (Inh2) rescues LRRK2‐G2019S‐dependent dopaminergic phenotypes. Strikingly, a holistic analysis of all studied features shows that the genetic background of the PD patients, and not the LRRK2‐G2019S mutation, constitutes the strongest contribution to the phenotypes. These data support the use of advanced in vitro models for future patient stratification and personalized drug development. [less ▲]

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See detailAdvanced Good Cell Culture Practice for human primary, stem cell-derived and organoid models as well as microphysiological systems
Pamies, David; Bal-Price, Anna; Chesné, Christophe et al

in ALTEX : Alternativen zu Tierexperimenten (2018)

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See detailActivity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity
Jan, Asad; Jansonius, Brandon; Delaidelli, Alberto et al

in Acta Neuropathologica Communications (2018)

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See detailMillifluidic culture improves human midbrain organoid vitality and differentiation
Berger, Emanuel UL; Magliaro, Chiara; Paczia, Nicole UL et al

in Lab on a Chip - Miniaturisation for Chemistry and Biology (2018)

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See detailNuclear localization and phosphorylation modulate pathological effects of Alpha-Synuclein
Pinho, Raquel; Paiva, Isabel; Jerčić, Kristina Gotovac et al

in Human Molecular Genetics (2018)

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See detailParkinson’s Disease-Associated Mutant LRRK2-Mediated Inhibition of miRNA Activity is Antagonized by TRIM32
Gonzalez-Cano, Laura; Menzl, Ingeborg; Tisserand, Johan UL et al

in Molecular Neurobiology (2017)

Detailed reference viewed: 113 (4 UL)