References of "Muller, Claude P"
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See detailFunctionally Convergent B Cell Receptor Sequences in Transgenic Rats Expressing a Human B Cell Repertoire in Response to Tetanus Toxoid and Measles Antigens.
Burckert, Jean-Philippe; Dubois, Axel R. S. X.; Faison, William J. et al

in Frontiers in immunology (2017), 8

The identification and tracking of antigen-specific immunoglobulin (Ig) sequences within total Ig repertoires is central to high-throughput sequencing (HTS) studies of infections or vaccinations. In this ... [more ▼]

The identification and tracking of antigen-specific immunoglobulin (Ig) sequences within total Ig repertoires is central to high-throughput sequencing (HTS) studies of infections or vaccinations. In this context, public Ig sequences shared by different individuals exposed to the same antigen could be valuable markers for tracing back infections, measuring vaccine immunogenicity, and perhaps ultimately allow the reconstruction of the immunological history of an individual. Here, we immunized groups of transgenic rats expressing human Ig against tetanus toxoid (TT), Modified Vaccinia virus Ankara (MVA), measles virus hemagglutinin and fusion proteins expressed on MVA, and the environmental carcinogen benzo[a]pyrene, coupled to TT. We showed that these antigens impose a selective pressure causing the Ig heavy chain (IgH) repertoires of the rats to converge toward the expression of antibodies with highly similar IgH CDR3 amino acid sequences. We present a computational approach, similar to differential gene expression analysis, that selects for clusters of CDR3s with 80% similarity, significantly overrepresented within the different groups of immunized rats. These IgH clusters represent antigen-induced IgH signatures exhibiting stereotypic amino acid patterns including previously described TT- and measles-specific IgH sequences. Our data suggest that with the presented methodology, transgenic Ig rats can be utilized as a model to identify antigen-induced, human IgH signatures to a variety of different antigens. [less ▲]

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See detailThe chromosome 15q14 locus for bipolar disorder and schizophrenia: Is C15orf53 a major candidate gene?
Kranz, Thorsten M.; Ekawardhani, Savira; Lin, Michelle K. et al

in Journal of Psychiatric Research (2012), 46(11), 1414-1420

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See detailEpigenetic regulation of the glucocorticoid receptor promoter 1 7 in adult rats
Witzmann, Simone UL; Turner, Jonathan D.; Mériaux, Sophie B. et al

in Epigenetics : Official Journal of the DNA Methylation Society (2012), 7(11), 1290-1301

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See detailInterplay of measles virus with early induced cytokines reveals different wild type phenotypes.
Kessler, Julia UL; Kremer, Jacques R.; Muller, Claude P.

in Virus Research (2011), 155(1), 195-202

Differential effects of measles virus (MV) on the innate immune response may influence virus spread and severity of disease. Using a representative panel of 22 MV strains including 14 different genotypes ... [more ▼]

Differential effects of measles virus (MV) on the innate immune response may influence virus spread and severity of disease. Using a representative panel of 22 MV strains including 14 different genotypes, we found that wild-type (wt) differ considerably in their sensitivity to type I interferon (IFN). The wt virus production was 2-47-fold lower in IFN-alpha treated Vero/hSLAM cells, whereas vaccine virus production was reduced only 2-3-fold. Sequence analysis of the MV-P/C/V gene, revealed no obvious amino acid mutations that correlated with the different phenotypes. Strains also widely differed in their ability to induce type I IFN, tumor necrosis factor (TNF) alpha and other cytokines in human A549/hSLAM cells. Some wt strains that were highly sensitive to type I IFN induced only low levels of these and other cytokines. In vitro wt strains that produced the 5' copy-back defective interfering RNAs (5'cb-diRNA) characterized by Shingai et al. (2007), induced high levels of cytokines that otherwise were only reached by vaccine strains. These 5'cb-diRNAs emerged only in virus cultures during multiple passaging and were not detectable in clinical samples of measles patients. These subgenomic RNAs are an important confounding parameter in passaged wt viruses which must be carefully assessed in all in vitro studies. The present data show that MV wt strains differ in their sensitivity and their ability to temper with the innate immune response, which may result in differences in virulence. [less ▲]

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See detailDecreased expression of mineralocorticoid receptor mRNA and its splice variants in postmortem brain regions of patients with major depressive disorder
Klok, Melanie D.; Alt, Simone UL; Irurzun Lafitte, Alicia J. et al

in Journal of Psychiatric Research (2011), 45(7), 871-78

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See detailRevealing new measles virus transmission routes by use of sequence analysis of phosphoprotein and hemagglutinin genes.
Kessler, Julia UL; Kremer, Jacques R.; Shulga, Sergey V. et al

in Journal of Clinical Microbiology (2011), 49(2), 677-83

With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a ... [more ▼]

With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-HVR sequences. However, these strains showed considerable diversity within a larger sequencing window based on concatenated MV phosphoprotein and hemagglutinin genes (P/H pseudogenes). In Belarus, Germany, Russia, and the Democratic Republic of Congo, the P/H pseudogenes provided insights into chains of transmission, whereas identical NP-HVR provided none. In Russia, for instance, the P/H pseudogene identified temporal clusters rather than geographical clusters, demonstrating the circulation and importation of independent variants rather than large local outbreaks lasting for several years, as suggested by NP-HVR. Thus, by extending the sequencing window for molecular epidemiology, a more refined picture of MV circulation was obtained with more clearly defined links between outbreaks and transmission chains. Our results also suggested that in contrast to the P gene, the H gene acquired fixed substitutions that continued to be found in subsequent outbreaks, possibly with consequences for its antigenicity. Thus, a longer sequencing window has true benefits both for the epidemiological surveillance of measles and for the better monitoring of viral evolution. [less ▲]

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See detailImproving global virologic surveillance for measles and rubella.
Rota, Paul A.; Brown, Kevin E.; Hubschen, Judith M. et al

in Journal of Infectious Diseases (2011), 204 Suppl 1

An important aspect of laboratory surveillance for measles and rubella is the genetic characterization of circulating wild-type viruses to support molecular epidemiologic studies and to track transmission ... [more ▼]

An important aspect of laboratory surveillance for measles and rubella is the genetic characterization of circulating wild-type viruses to support molecular epidemiologic studies and to track transmission pathways. Virologic surveillance that is sufficient to document the interruption of transmission of measles and rubella viruses will be an essential criterion for verification of elimination. Laboratories in the World Health Organization (WHO) Measles and Rubella Laboratory Network have worked to improve and expand virologic surveillance as many regions move toward elimination of measles and rubella/congenital rubella syndrome. As countries approach elimination, it will be necessary to obtain genetic information from as many chains of transmission as possible. In addition, baseline virologic surveillance, especially for rubella, needs to be improved in many countries. This report contains a summary of recent improvements to the methods used for virologic surveillance. [less ▲]

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See detailStress induces methylation of the E2F1 regulated glucocorticoid receptor promoter 1F
Alt, Simone UL; Cao, Lei; Leija, Salomon C. et al

Scientific Conference (2010)

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See detailTranscriptional control of the glucocorticoid receptor: CpG islands, epigenetics and more
Turner, Jonathan D.; Alt, Simone UL; Cao, Lei et al

in Biochemical Pharmacology (2010), 80(12), 1860-68

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See detailDifferential expression of glucocorticoid receptor transcripts in major depressive disorder is not epigenetically programmed
Alt, Simone UL; Turner, Jonathan D.; Klok, Melanie D. et al

in Psychoneuroendocrinology (2010), 35(4), 544-56

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See detailMineralocorticoid receptor is downregulated in hippocampus and cingulate gyrus of depressed patients; evidence for a MR/GR imbalance
Irurzun-Lafitte, Alicia J.; Klok, Melanie D.; Alt, Simone UL et al

Scientific Conference (2009)

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See detailAlternative glucocorticoid receptor first exon usage in major depressive disorder
Alt, Simone UL; Turner, Jonathan D.; Klok, Melanie D. et al

Scientific Conference (2008)

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See detailChanges of Glucocorticoid receptor first exon variants in major depressive disorder
Alt, Simone UL; Turner, Jonathan D.; Klok, Melanie D. et al

Scientific Conference (2008)

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See detailNuclear receptors in human immune cells: expressions and correlations
Muller, Claude P.; Schiltz, Jang UL; Turner, Jonathan D. et al

in Molecular Immunology (2007), 44

Nuclear receptors (NR) are key modulators of gene transcription. Their activity is ligand induced and modulates a large variety of tissue-specific cellular functions. However, for many NR little is known ... [more ▼]

Nuclear receptors (NR) are key modulators of gene transcription. Their activity is ligand induced and modulates a large variety of tissue-specific cellular functions. However, for many NR little is known about their role in cells of the immune system. In this study, expression patterns and distribution of 24 NR were investigated in human peripheral blood mononuclear cells.We provide the first evidence of the expression of the 12 receptors CAR, CoupTF , CoupTF , FXR, GCNF, HNF4 , PPAR / , PXR, RevErb , TR2, TR4 and TLX in highly purified CD4, CD8, CD19, CD14 cells. The expression profile of RevErb and LXR previously observed in B cell and macrophages, respectively, has been extended to CD4, CD8 and CD14 cells. Except for RAR , which was absence in any of the cells tested, our results suggest an almost ubiquitous expression of the NR in the different cell lineages of the immune system. The expression of CAR, CoupTF , FXR was also confirmed at a protein level and despite conspicuous mRNA levels of HNF4 , only low levels of this receptor were detectable in the nuclear fraction of PBMCs. Expression of the latter receptors was mostly only a fraction (4–20%) of their expression in the thyroid gland, the adrenal gland, the lung or subcutaneous adipose tissue. The Spearman rank order correlation test was performed to examine the correlation in expression between individual nuclear receptor pairs in the four cell types for several donors. Distinct correlation patterns were observed between receptor pairs in the individual cell types. In CD4 T cells four NR, GCNF, PPAR , PPAR 7 and RevErb are perfectly correlated with each other (P≥0.0167). In the other cell types correlations betweenNRpairs were more diverse, but also statistically highly significant. Interestingly, the relative expression level of a number of receptor pairs ranked identical or similar in at least three (CoupTF and PPAR / , CoupTF andHNF4 as well asROR and PXR) or four cell types (CoupTF and CoupTF , PPAR and RevErb ). Despite the variability of NR expression in immune cells, these results suggest that some of the NR may be co-regulated in human immune cells. [less ▲]

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