References of "Messiha, Hanan L"
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See detailA model of yeast glycolysis based on a consistent kinetic characterisation of all its enzymes
Smallbone, Kieran; Messiha, Hanan L.; Carroll, Kathleen M. et al

in FEBS Letters (2013), 587(17), 2832-2841

We present an experimental and computational pipeline for the generation of kinetic models of metabolism, and demonstrate its application to glycolysis in Saccharomyces cerevisiae. Starting from an ... [more ▼]

We present an experimental and computational pipeline for the generation of kinetic models of metabolism, and demonstrate its application to glycolysis in Saccharomyces cerevisiae. Starting from an approximate mathematical model, we employ a ‘‘cycle of knowledge’’ strategy, identifying the steps with most control over flux. Kinetic parameters of the individual isoenzymes within these steps are measured experimentally under a standardised set of conditions. Experimental strategies are applied to establish a set of in vivo concentrations for isoenzymes and metabolites. The data are integrated into a mathematical model that is used to predict a new set of metabolite concentrations and reevaluate the control properties of the system. This bottom-up modelling study reveals that control over the metabolic network most directly involved in yeast glycolysis is more widely distributed than previously thought. [less ▲]

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See detailBuilding a kinetic model of trehalose biosynthesis in Saccharomyces cerevisiae.
Smallbone, Kieran; Malys, Naglis; Messiha, Hanan L. et al

in Methods in Enzymology (2011), 500

In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by ... [more ▼]

In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast. The biology of the trehalose cycle is briefly reviewed and discussed. [less ▲]

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